Figure 4.

Behavioral responses of tethered BmPBP1-knockout moths to intermittent pheromone pulses

(A) Schematic diagram of the pheromone-triggered programmed behavior and pheromone source localization strategy of a male silkmoth, B. mori. The dots denote pheromone filaments. Note that the actual behavior during zigzagging and looping consists of point turns.

(B) Analysis of pheromone-triggered programmed behavior in BmPBP1-knockout (blue; n = 9) and wild-type (red; n = 16) male moths to single-pulse bombykol stimulation (1 s duration). Error bars represent ±SEM. The asterisks indicate significant differences between the groups (∗∗p < 0.01, Student’s t test for paired samples). NS indicates no significant difference.

(C) Representative trace of the body angle of tethered BmPBP1-knockout (blue) and wild-type male (red) moths to pulsed bombykol stimuli at 0.17 and 2 Hz.

(D) Surge rate in BmPBP1-knockout (blue) and wild-type (red) male moths to bombykol pulse trains at 0.17 Hz (KO; n = 9, WT; n = 16), 0.83 Hz (KO; n = 10, WT; n = 7), and 2 Hz (KO; n = 9, WT; n = 9). Error bars represent ±SEM. The asterisks indicate significant differences between the groups (∗∗p < 0.01, Student’s t test for paired samples). NS indicates no significant difference.

(E) Scatterplot of each surge based on surge duration in BmPBP1-knockout (blue; n = 9) and wild-type (red; n = 9) male moths. Circles indicate the distribution of individual data. Solid circles indicate the first surge that was induced by the first bombykol stimuli.

See also Figure S2.