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A) Neither treatment was able to modulate astrogliosis, B) but C2C12 conditioned medium administration increased the dispersion of cells from the slice edges (as demonstrated by the numerous DAPI positive nuclei located over the edge of the slice): C) among the migrating cells, we mainly observed microglial (IBA1 positive) and D) astrocytes (GFAP positive). DAPI was used to label the cell nuclei. Scale bar: A,B) 500 μm; C,D) 50 μm.
