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Multicopy suppression of temperature restriction. (a) Equal amounts of genomic DNA from strain CEM-8 [RIM101/(RIM101-1426)n≥3], CEM-7 (RIM101/RIM101-1426/RIM101-1426), and the parental strain CEM-1 (RIM101/RIM101-1426) were digested with either PstI (left panel) or EcoRI (right panel). The Southern blots were hybridized with RIM101 or ACT1, as indicated. (b) Morphology of CEM-1, CEM-7, and CEM-8 following 3 h of incubation in medium 199 at pH 4.0 or pH 7.0 and 29 or 37°C.
