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. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: Biochemistry. 2021 Jun 3;60(23):1797–1807. doi: 10.1021/acs.biochem.1c00130

Figure 2:

Figure 2:

Primer extension assays for replication bypass of covalent DNA–peptide cross-links by hPol η. 32P-labeled 12-mer primer was annealed with 17-mer DNA containing 5-formyl dC conjugated to an 11-mer peptide (X=DpC) (top panel) or unmodified dC (X=dC) (bottom panel) via oxime ligation. YXZ corresponds to the sequence context used, as listed above the X-axis. Z’ corresponds to the nucleotide complementary to Z in each sequence context. Primer extension reactions were initiated by the addition of hPol η and a mixture of dNTPs. Reactions were quenched at specific time points (0, 5, 15, 30 min), and loaded onto a 20% PAGE gel containing 7M urea.