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. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: Biochemistry. 2021 Jun 3;60(23):1797–1807. doi: 10.1021/acs.biochem.1c00130

Table 1:

Nucleobase sequence and mass spectrometry characterization of synthetic DNA/DpC oligodeoxynucleotides.

Sequence Expected mass (Da) Observed mass (Da)
A 5′-d(TTC CFC GTC ACG ACG TT)-3′ 5125.4 5124.6
B 5′-d(TTC CFG GTC ACG ACG TT)-3′ 5165.4 5164.5
C 5′-d(TTC CFT GTC ACG ACG TT)-3′ 5140.4 5139.6
D 5′-d(TTC AFA GTC ACG ACG TT)-3′ 5173.4 5172.6
E 5′-d(TTC GFA GTC ACG ACG TT)-3′ 5189.4 5188.6
F 5′-d(TTC TFA GTC ACG ACG TT)-3′ 5164.4 5163.6
G 5′-d(TTC CFA GTC ACG ACG TT)-3′ 5149.3 5149.4
H 5’-d(TTC CXC GTC ACG ACG TT)-3’ 5967.2 5966.2
I 5’-d(TTC CXG GTC ACG ACG TT)-3’ 6007.3 6006.2
J 5’-d(TTC CXT GTC ACG ACG TT)-3’ 5982.2 5981.6
K 5’-d(TTC AXA GTC ACG ACG TT)-3’ 6015.3 6014.5
L 5’-d(TTC GXA GTC ACG ACG TT)-3’ 6031.3 6030.0
M 5’-d(TTC TXA GTC ACG ACG TT)-3’ 6006.3 6005.0
N 5’-d(TTC CXA GTC ACG ACG TT)-3’ 5989.2 5988.2
F

=5-formyl-dC

X

=5-formyl-dC crosslinked to NH2-GGG KGL GK*G GA, where K*=oxy-lysine