Figure 2.

Effects of CA on lipofuscin and reproduction of C. elegans. Wild-type worms were treated with DMSO (control) or with rapamycin (2 nM) or CA (5, 10, 20 μM) for 5 days, followed by detection of lipofuscin autofluorescence as shown by the representative images of worms, scale bar = 100 μm. (A) Cumulative data are shown in the bar figure (B) n > 19 worms per group, ** p = 0.0016, *** p = 0.0001, **** p < 0.0001). Egg production rates were recorded (C) n = 10 worms per group, p > 0.05).