TABLE 3.
Molecular characterization of ESBL-producing E. coli and K. pneumoniae isolates
Patient | Isolate | SHV | TEM | Macrorestriction genotypea | Plasmid digestion profileb | β-Lactamase pIc |
---|---|---|---|---|---|---|
P1 | kp1 | SHV-2 | B | ND | 7.6 | |
P1 | kp2 | SHV-2 | B | ND | 7.6 | |
P2 | kp3 | SHV-2 | B | ND | 7.6 | |
P3 | kp4 | SHV-2 | B | ND | 7.6 | |
P4 | kp5 | SHV-5 | E | e | 8.2 | |
P5 | kp6 | SHV-5 | F | f | 8.2 | |
P6 | kp7 | SHV-5 | G | g | 8.2 | |
P7 | kp8 | SHV-5 | H | h | 8.2 | |
P8 | kp9 | SHV-5 | TEM-1 | I | i | 5.4, 8.2 |
P9 | kp10 | SHV-2 | J | j | 7.6 | |
P9 | kp11 | SHV-5 | K | k | 8.2 | |
P10 | kp12 | SHV-5 | TEM-1 | L | l | 5.4, 8.2 |
P11 | ec13 | SHV-5 | TEM-1 | A | a | 5.4, 8.2 |
P11 | ec14 | SHV-5 | TEM-1 | A | c | 5.4, 8.2 |
P12 | ec15 | SHV-5 | TEM-1 | C | b | 5.4, 8.2 |
P13 | ec16 | SHV-5 | TEM-1 | D | d | 5.4, 8.2 |
Macrorestriction genotypes were determined by PFGE after digestion with XbaI.
Plasmid digestion profile for tranconjugants digested with PvuII and PstI. ND, not determined.
β-Lactamase pIs were determined by isoelectric focusing.