. 2021 Oct 30;26(21):6583. doi: 10.3390/molecules26216583

Table 2.

Effect of compounds 13a–c and 14a–c on Ca²⁺ mobilization in FPR-transfected HL60 cells.

graphic file with name molecules-26-06583-i002.jpg

Compound	R	R₁	R₂	FPR1-HL60 EC₅₀ (μM) and efficacy (%) ^a	FPR2-HL60 EC₅₀ (μM) and efficacy (%) ^a	hPMN EC₅₀ (μM) and efficacy (%) ^a
13b	H	OH	OCH₃	N.A. ^b	N.A. ^b	N.A. ^b
13c	OH	H	OCH₃	N.A. ^b	N.A. ^b	N.A. ^b
14a	Br	H	OH	0.23 ± 0.06 (115)	0.23 ± 0.02 (150)	3.7 ± 0.7 (130)
14b	H	OH	OH	40.1 ± 7.2 (50)	37.6 ± 5.6 (150)	N.A. ^b
14c	OH	H	OH	N.A. ^b	N.A. ^b	N.A. ^b
fMLF				0.01
WKYMVM					0.001
AMC3 [24]				1.6 ± 0.01	0.12 ± 0.02	0.39 ± 0.17
AMC4 [24]				3.0 ± 0.4	0.38 ± 0.09	0.83 ± 0.24

^a EC₅₀ values represent the mean of three independent experiments and were determined by nonlinear regression analysis of the concentration-response curves (5–6 points) generated using GraphPad Prism 9 with 95% confidential interval (p < 0.05). ^b N.A. no activity (no response was observed during first the 2 min after addition of the compounds under investigation) considering the limits of efficacy < 20% and EC₅₀ < 50 µM.