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Endosomal escape of Lipo2000@siRNA-Cy5 (A–C) and GNS-P@siRNA-Cy5 (D–F) for 4 h, 8 h and 12 h by CLSM. The nucleus was stained with Hoechst 33342 (blue signal), siRNA was labeled by Cy5 (red signal) and the endosome was stained with LysoTracker Green (green signal). (A1–F1), overlay of endosome and nucleus; (A2–F2), overlay of siRNA and nucleus; (A3–F3), overlay of endosome and siRNA; (A4–F4), overlay of endosome, siRNA and nucleus. The scale bar was 20 μm.
