Table 3.

Accumulation of mangiferin analysed by HPLC-UV among skin layers: stratum corneum, epidermis, and dermis, and mangiferin permeation through the skin after 2, 4, 6, and 24 h (ng/cm²) from the application of mangiferin solutions (MH₂O, MEtOH) or honeybush extracts (A, B, C, D).

	MH2O	MEtOH	Extract A	Extract B	Extract C	Extract D
S.c.	32.62 ± 4.61	29.92 ± 2.07	24.46 ± 2.36	29.44 ± 7.51	56.92 ± 3.72	26.92 ± 1.53
Epidermis	41.62 ± 7.45	32.08 ± 1.97	32.74 ± 2.45	48.51 ± 7.08	74.26 ± 5.95	32.72 ± 2.74
Dermis	53.32 ± 16.41	35.23 ± 4.73	23.95 ± 2.15	29.13 ± 1,84	58.67 ± 2.67	24.1 ± 1.97
2 h	30.23 ± 5.22	48.85 ± 7.37	30.97 ± 1.54	33.85 ± 5.31	61.03 ± 5.05	34.1 ± 7.44
4 h	35.38 ± 5.9	59.08 ± 6.76	39.95 ± 1.72	42.31 ± 8.18	82.36 ± 7.27	43.28 ± 6.74
6 h	40.54 ± 7.77	69.15 ± 7	49.23 ± 2.66	50.92 ± 7.52	111.54 ±9.39	56.56 ± 4.66
24 h	66.15 ± 11.92	77.78 ± 8.38	59.33 ± 3.76	62.82 ± 9.46	152.36 ± 8.57	75.69 ± 2.14

S.c. stratum corneum; each average value was obtained from three independent repetitions.