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. 2021 Nov 5;26(21):6688. doi: 10.3390/molecules26216688

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Modulation of CB2 receptor expression in MDA-MB-468 cells detected after 24 h treatment with 10 μg/mL FOS (organic extract from Felina 32 hemp inflorescences collected in September), the corresponding mFOS cocktail, and with the nonintoxicating phytocannabinoids and caryophyllane sesquiterpenes contained in FOS extract. (A) Representative images obtained at immunofluorescence analysis. (B) Densitometric analysis of immunofluorescence carried out by the free software ImageJ (n = 6). ** p < 0.01 and *** p < 0.001, significant difference respect to the control (ANOVA + Dunnett’s Multiple Comparison PostTest). 1. Control; 2. FOS; 3. mFOS; 4. Cannabidiol, 3.4 μg/mL; 5. Cannabichromene, 1.2 μg/mL; 6. α-Humulene, 0.02 μg/mL; 7. β-Caryophyllene, 0.02 μg/mL; 8. β-Caryophyllene oxide, 0.02 μg/mL.