Figure 2.

HPLC-DAD chromatogram of MeOH/H₂O 80% extract of R. gallica var. aegyptiaca leaves. The figure shows the chromatographic separation of phenolic compounds based on the following conditions: Column, Zorbax C₁₈, 5µm, 280 × 4.6 mm i.d.; detection at 350, 325, and 280 nm; flow rate, 0.5 mL/min; gradient elution system of methanol/water/formic acid; injected volume, 10 µL. Peaks: (1) gallic acid; (2) (+) catechin; (3) chlorogenic acid; (4) (–) epicatechin; (5) quercetin-3-O-α-d -(glucopyranoside); (6) quercetin; (*) unknown peaks.