PCR-RFLP of B. henselae, B. vinsonii, B. elizabethae, and B. quintana. Lane 1, 50-bp ladder; lanes 2 to 4, B. henselae B91-002000; lanes 5 to 7, B. vinsonii B92-010225; lanes 8 to 10, B. elizabethae B92-002005; lanes 11 to 13, B. quintana ATCC VR-358; lane 14, 50-bp ladder. PCR products were uncut (lanes 2, 5, 8, and 11) or cut with DdeI (lanes 3, 6, 9, and 12) or MseI (lanes 4, 7, 10, and 13).