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. 2021 Nov 10;9(1):e1107. doi: 10.1212/NXI.0000000000001107

Figure 4. Expression of CD59 Protects Against IgM Anti–GM1-Induced Complement-Mediated Cell Lysis.

Figure 4

(A) sNF96.2 cells express GM1 and CD59. Pretreatment of SCs with phospholipase C reduces expression of GPI-linked CD55 and CD59, but not of transmembrane CD46 or GM1. (B) MMN patient-derived IgM antibodies bind to GM1-expressing sNF96.2 cells. GM1 staining is shown in green and IgM staining in red. Images were obtained using a Zeiss Z1 microscope (Carl Zeiss Microscopy) with Colibri LEDs with the following settings: ×20 magnification, 25% LED, 400 ms for Alexa FluorTM 488 channel, 100 ms for APC channel, and 50 ms for 4'6- diamidino-2-phenylindole channel. (C) On activation with heat-inactivated MMN serum and fresh serum, SCs with intact CD55 and CD59 expression display no lysis, as measured with Annexin V and 7-AAD staining, C3 fixation (D), or MAC insertion (E). Data represent mean ± SEM of 3 independent experiments. MAC = membrane attack complex; MMN = multifocal motor neuropathy; MN = motor neuron.