Figure 2.

Correlative 3D-SMLM and IRM within the immunological synapse. (a) Experiments were performed on a T-cell adhering to an SLB functionalized with I-E^k/MCC, B7-1 and ICAM-1; the TCR was labeled via AF647-conjugated H57-scFv. (b) IRM (left) and SMLM/IRM overlay images (right) of the immunological synapse. The areas i, ii, and iii are shown in magnification below. (c) Correlation plots between z_SMLM and the normalized IRM intensity for the areas indicated in panel b. Data points with z_SMLM < 100 nm were fitted with SI Materials and Methods eq 3 (solid line). The dashed lines indicate the square root of the Cramér-Rao lower bound. Scale bars 2 μm.