TABLE 1.
List of antibodies used for Immunofluorescence Staining (IF) or immunocytochemistry (ICC).
| Primary antibodies | ||||||
| Immunogen | Host species | Dilution | Manufacturer | Catalog no. | Application | |
|
| ||||||
| Iba-1 | Rabbit | 1:500 | Wako | 019-19741 | IF | |
| GFAP | Rabbit | 1:500 | Neuromics | RA22101 | IF | |
| NLRP3 | Goat | 1:100 | Abcam | AB4207 | IF, ICC | |
| COX-2 | Rabbit | 1:100 | Cell Signaling | 89332 | IF | |
| pERK | Rabbit | 1:200 | Cell Signaling | 9101 | ICC | |
| IL-6 | Mouse | 1:50 | Santa Cruz | SC-57315 | IF | |
| CD11b | Rat | 1:200 | Abcam | AB8878 | ICC | |
| GFAP | Chicken | 1:500 | Millipore | AB5541 | ICC | |
| p-STAT3S727 | Rabbit | 1:200 | Abcam | AB86340 | ICC | |
| p-NF-κBS536 | Rabbit | 1:200 | Cell Signaling | 3033S | ICC | |
|
| ||||||
| Secondary antibodies | ||||||
|
| ||||||
| Antibody | Dilution | Manufacturer | Catalog no. | Application | ||
|
| ||||||
| Goat anti-rabbit IgG, 555 | 1:200 | Invitrogen | A21428 | IF | ||
| Goat anti-rabbit IgG, 488 | 1:200 | Invitrogen | A11008 | IF, ICC | ||
| Goat anti-mouse IgG, 488 | 1:200 | Invitrogen | A11001 | IF | ||
| Donkey anti-goat, 555 | 1:200 | Invitrogen | A21432 | IF | ||
| Goat anti-chicken IgG, 488 | 1:200 | Abcam | A150169 | IF | ||
| Goat anti-rat IgG, FITC | 1:200 | Invitrogen | A18866 | ICC | ||