FIG. 1.

Schematic diagram of the positive regulatory regions (PRRs) of the 5′ proximal promoter of the human IL-2Rα gene. Shown is the promoter region between nucleotides −472 and +158, indicating the binding sites for previously identified transcription factors involved in regulating gene expression in vivo (23, 24). Note that nt +1 is the more 3′ of the two major identified transcription start sites for this gene. (A) PCR primer locations and restriction enzyme cut sites used in this study are indicated. (B) Sequences of the wt (A₁₈) and mutant (R₁₈) PRRII promoter DNAs, with hatched boxes indicating the binding sites for HMG-I(Y) and Elf-1 of the wt promoter (23).