Figure 2.

Influence of the miR-181a-2 on the cell sensitivity to tamoxifen. The single (a,b) or multiple (c) transfection of MCF7 cells with miR-181a-2 mimetic or scrambled RNA construct as control was performed as described in Methods. To determine the cell sensitivity to tamoxifen the cells in one day (a), five days (b) after single transfection or the cells cultured for two months after multiple transfections (c) were treated with tamoxifen for 72 h and the number of the viable cells was counted by the MTT test. Data represent the mean value of three independent experiments. * p < 0.05 versus scrambled (scr).