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. 2021 Oct 20;26(21):6339. doi: 10.3390/molecules26216339

Figure 2.

Figure 2

Quantitative FRET signal analysis: (A). Fluorescent emission of FRET donor and acceptor mixture at 530 nm, when excited at 414 nm (FLDA), can be divided into three components: FRET emission from donor, direct emission of donor, and direct emission of acceptor; (B). The cross-wavelength correlation coefficiency of donor α is defined as the fluorescent emission of donor at 530 nm, when excited at 414 nm (FLDA), divided by the fluorescent emission of donor at 475 nm, when excited at 475 nm (FLAA); (C). The cross-wavelength correlation coefficiency β of the acceptor is defined as the fluorescent emission of donor at 530 nm, when excited at 414 nm (FLAD), divided by the fluorescent emission of donor at 530 nm, when excited at 475 nm (FLAA).