TABLE 3.
Tissue distribution of parasite DNA and comparison of nested and standard PCR methods for detection of N. caninum PCR
| Tissue type | No. of tissue samples tested | No. (%) of samples positive by:
|
|
|---|---|---|---|
| PCR | Nested PCR | ||
| Fixed | |||
| Brain | 13 | 13 (100) | 13 (100) |
| Lung | 12 | 6 (50) | 11 (92) |
| Placenta | 5 | 2 (40) | 5 (100) |
| Kidney | 13 | 5 (38) | 11 (85) |
| Liver | 12 | 3 (25) | 10 (83) |
| Heart | 13 | 3 (23) | 8 (62) |
| Spleen | 12 | 2 (17) | 7 (58) |
| Fresh | |||
| Kidney | 3 | 3 (100) | 3 (100) |
| Brain | 8 | 7 (88) | 8 (100) |
| Heart | 5 | 3 (60) | 4 (80) |
| Lung | 5 | 2 (40) | 5 (100) |
| Liver | 7 | 2 (29) | 3 (43) |
| Placenta | 1 | 0 (0) | 1 (100) |
| Spleen | 2 | 0 (0) | 1 (50) |