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. 2021 Nov 8;26(21):6740. doi: 10.3390/molecules26216740

Figure 1.

Figure 1

Comparison of detergent-free with detergent-based decellularization of porcine pancreata. (A) Porcine pancreatic tissues before (pPan) and after decellularization using a detergent-based (DB) or detergent-free method (DFB). (B) DNA content of pPan (n = 6), detergent-based (n = 3) or detergent-free (n = 6) dpECM. *: p < 0.05; NS: not significant. (C) H&E staining showing porcine pancreatic tissue morphology before (pPan) and after decellularization (DFB) with a detergent-free method. Scale bars: 100 μm. (D) Average numbers of extracellular proteins identified from detergent-based or detergent-free dpECM. *: p < 0.05. (E) Venn diagram showing the total numbers of extracellular proteins identified in the dpECM. (F–H) Gene ontology enrichment analysis of extracellular proteins identified in the dpECM assigned to biological process (F), molecular function (G), and cellular component (H). *: p < 0.05; **: p < 0.01; ***: p < 0.001. Data are shown as mean ± SD.