Table 1.

Fractions obtained in experiments carried out for three plant species of the Lycopodiaceae family using different combinations of sorbents and different mobile phases using the VLC method.

The Number of Experiments	1			2			3		4
Solvent gradient system used	95:5:0.2 (v/v/v) chloroform: methanol: 25% aqueous ammonia solution								60% aqueous methanol solution with 2 drops of 10% aqueous tartaric acid 85% aqueous methanol solution with 2 drops of 10% aqueous tartaric acid
Sorbent filling ratio	1:3 Al2O3 (17 g):silica gel (25 g)			3:1 Al2O3 (57 g):silica gel (8 g)			silica gel 60 H silanized (26 g)		silica gel 60 H silanized (26 g)
Plant materials	LCL	LAN	HS	LCL	LAN	HS	LCL	LAN	LCL
Number of fractions obtained	6	14	18	10	11	16	7	11	11

LCL—Lycopodium clavatum L., LAN—Lycopodium annotinum L., HS—Huperzia selago L.