Table 2.
Results of the alkaloids identified via HPLC/ESI-QTOF-MS present in fractions 1-14 obtained from experiment 1 via VLC using sorbents Al2O3 and silica gel in ratio 1:3 from L. annotinum extract.
| Isolated Compounds | Fraction Number | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |
| Acrifoline | - | - | - | - | - | 4.5 | - | - | 12.6 | 16.0 | 20.7 | 22.7 | 21.6 | 6.4 |
| Lycopodine | - | - | - | - | - | 22.1 | 28.6 | 22.9 | 16.8 | 23.5 | 17.3 | 21.0 | 8.1 | - |
| Annotinine | - | 85.7 | 52.6 | 9.8 | - | - | - | - | - | - | - | - | - | - |
| Dihydroannotine | - | - | - | - | - | - | - | - | - | 15.0 | 26.0 | 29.1 | 12.5 | - |
| Lycodine | - | 1.6 | 14.6 | 28.5 | 32.2 | 25.9 | 28.4 | 7.0 | 3.1 | 0.7 | - | - | - | - |
| Lyconnotine | - | - | - | 6.1 | 7.8 | - | - | - | - | - | - | - | 41.9 | - |
| Annotine N-oxide | - | - | 16.8 | 41.3 | 14.3 | 14.6 | 12.4 | - | - | - | - | - | - | 22.3 |
| Huperzinine | - | - | - | 2.1 | 1.7 | 1.6 | 1.8 | - | - | - | - | - | - | - |
| Lyconadine A | - | - | - | 2.3 | 1.9 | 2.5 | 2.8 | 1.2 | 0.9 | - | - | - | - | - |
| Lycopodine N-oxide | - | - | - | - | - | 59.8 | 56.8 | 38.5 | 31.9 | 23.5 | 3.1 | |||
| Acetylfawcettiine | - | - | - | - | - | - | - | - | - | - | - | - | - | 4.8 |
| 8-β,11-α Aihydroxylycopodine | - | - | - | - | - | - | - | - | - | - | - | - | - | 43.4 |
| Des-N-methyl-α-obscurine | - | - | - | - | - | - | - | - | - | - | - | - | 4.5 | 5.5 |
| % SUM | - | 87.3 | 84.0 | 90.2 | 57.9 | 71.1 | 73.8 | 90.9 | 90.3 | 93.7 | 95.8 | 96.2 | 88.5 | 85.4 |