Table 3.
Results of alkaloids identified via HPLC/ESI-QTOF-MS present in fractions 1–11 obtained from experiment 2 via VLC using sorbents Al2O3 and silica gel in ratio 3:1 from L. annotinum extract.
| Isolated Compounds | Fraction Number | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | |
| Acrifoline | - | - | 1.9 | - | - | 2.0 | 17.4 | 25.1 | 18.9 | 14.5 | 3.2 |
| Lycopodine | - | - | 14.5 | - | 12.5 | 73.9 | 41.1 | 12.0 | - | - | - |
| Annotinine | 85.2 | 89.0 | 71.3 | 87.9 | 61.7 | - | - | - | - | 9.2 | 5.6 |
| Fawcettimine | - | - | - | - | - | - | - | 7.2 | 17.5 | 8.3 | - |
| Annotine | - | - | - | - | - | - | - | 14.4 | - | - | - |
| Dihydroannotine | - | - | - | - | - | - | - | 8.1 | 16.6 | - | 4.5 |
| Dihydroannotinol | - | - | - | - | - | - | - | 6.5 | 11.6 | - | 4.7 |
| N-Methyl dihydrolycodine | - | - | - | - | - | - | - | 6.9 | 6.9 | 4.7 | 8.0 |
| Lycodine | 1.4 | 1.4 | 3.4 | 1.2 | 3.6 | 5.8 | 3.5 | - | - | - | - |
| Dihydrolycopodine | - | - | - | - | - | - | - | - | - | 18.1 | 7.0 |
| Deacetyllycofawcine | - | - | - | - | - | - | - | - | - | - | 3.2 |
| Lycopodine N-oxide | - | - | - | - | 4.6 | 5.8 | 28.0 | 9.1 | 18.4 | - | - |
| Deacetylfawcettiine | - | - | - | - | - | - | - | - | - | 9.2 | 26.3 |
| α-Obscurine | - | - | - | - | - | - | - | - | 1.5 | 4.1 | 2.2 |
| Lyconnotine | 8.2 | 2.6 | 3.8 | 6.2 | 4.7 | 6.1 | 8.4 | 7.2 | 7.9 | - | |
| Unidentified 474.3794 C26H52NO6 |
- | - | - | - | - | - | - | - | - | 22.7 | - |
| Unidentified 459.2649 C29H35N2O3 |
- | - | 4.7 | 6.1 | 6.2 | 6.4 | 2.1 | - | - | - | - |
| % SUM | 86.6 | 98.6 | 98.4 | 99.0 | 94.9 | 98.5 | 98.1 | 97.6 | 98.5 | 98.8 | 64.6 |