Table 4.
Results of alkaloids identified via HPLC/ESI-QTOF-MS present in fractions 1–11 obtained from experiment 3 via VLC using silanized silica gel as a sorbent from L. annotinum extract.
| Isolated Compounds | Fraction Number | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | |
| Acrifoline | - | 16.2 | 17.8 | 21.6 | 28.2 | 23.5 | 28.3 | 63.6 | 30.4 | - | 12.4 |
| Lycopodine | 0.9 | 15.8 | - | - | - | - | - | - | - | - | - |
| Annotinine | - | 27.4 | 43.4 | 34.3 | 30.2 | 36.6 | 31.1 | - | - | - | - |
| Annotine | - | - | - | - | - | 1.99? | 1.9 | - | - | - | - |
| Dihydroannotine | - | - | - | - | - | - | - | - | 7.6 | 18.8 | 11.8 |
| Fawcettimine | - | 9.9 | 8.7 | 13.7 | 11.4 | 13.5 | 15.8 | 13.7 | 34.1 | 11.5 | 6.7 |
| β-Obscurine | - | - | 1.4 | 2.3 | 1.1 | 1.3 | 1.7 | 1.2 | - | - | 2.8 |
| Lycopodine N-oxide | - | - | - | - | - | - | - | 4.1 | 7.4 | 9.2 | - |
| Lycodine | - | 3.0 | 1.5 | 1.6 | - | - | - | - | - | - | - |
| Deacetylfawcettiine | 14.0 | - | - | - | - | - | - | 5.3 | 1.8 | 14.9 | 4.5 |
| Lyconnotine | - | 15.8 | 19.3 | 18.5 | 21.0 | 16.1 | 11.6 | 3.2 | - | - | - |
| Huperzinine | - | - | - | - | - | - | - | - | 2.5 | 3.9 | 7.4 |
| N-Methyl lycodine | - | - | - | - | - | - | - | - | - | 5.5 | 14.1 |
| Flabelline | - | 3.5 | 2.3 | 4.2 | 2.1 | 2.0 | 1.5 | - | - | - | - |
| Unidentified 474.3794 C26H52NO6 |
12.6 | - | - | - | - | - | - | 4.6 | 11.8 | 14.2 | - |
| % SUM | 27.4 | 91.7 | 94.4 | 96.2 | 94.0 | 93.0 | 91.9 | 95.7 | 95.5 | 78.1 | 59.7 |