Table 5.
Results of alkaloids identified via HPLC/ESI-QTOF-MS present in fractions 1–18 obtained from experiment 1 via VLC using sorbents Al2O3 and silica gel in ratio 1:3 from H. selago extract.
| Isolated Compounds | Fraction Number | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | |
| Selagoline | - | - | - | - | - | - | - | - | - | 6.1 | 18.8 | 14.7 | 26.7 | 29.1 | 22.9 | 4.1 | 18.1 | |
| Lycopodine N-oxide | - | - | - | - | - | - | - | - | - | 12.0 | 24.7 | 38.7 | 27.0 | 26.4 | 22.0 | 4.1 | 20.6 | 35.6 |
| Anhydrolycodoline | 1.1 | 1.2 | 1.0 | 0.9 | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| 8-β,11-α Dihydroxy-lycopodine | - | - | - | - | - | - | - | - | - | - | - | - | - | - | 4.2 | 18.0 | 30.2 | |
| Lycoposerramine B | - | - | 2.2 | 1.8 | 1.2 | 1.9 | 1.8 | 1.7 | 3.2 | 1.2 | - | - | - | - | - | - | - | - |
| Huperzine A | - | - | - | - | - | - | - | - | - | - | 5.2 | 5.6 | 4.7 | 2.3 | 1.7 | - | - | - |
| 16-Hydroxyhuperzine B | - | - | - | - | - | - | - | - | - | - | - | - | - | - | 0.8 | 2.2 | 1.3 | - |
| Huperzine B | - | - | - | - | - | - | - | - | - | - | - | - | - | - | 4.5 | - | - | - |
| Lycodine | - | - | - | - | 3.1 | 5.3 | 5.6 | 4.8 | 4.2 | 4.1 | 2.2 | - | - | - | - | - | - | - |
| Des-N-methyl- β-obscurine | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | 5.4 | 4.6 | - |
| Dehydrooxolucidine B | 19.8 | 51.4 | 33.5 | 57.6 | 55.8 | 49.6 | 54.2 | 45.2 | 14.4 | 3.2 | 6.2 | 5.5 | 3.2 | 4.4 | - | - | - | |
| Serratidine | 2.0 | 4.6 | 4.3 | 3.9 | 2.1 | 0.9 | 1.1 | 1.3 | 1.2 | - | - | - | - | - | - | 1.3 | 1.5 | - |
| Lucidine B | 3.3 | 28.8 | 16.6 | 21.6 | 15.2 | 17.2 | 14.3 | 18.1 | 23.2 | 39.2 | 31.2 | 24.2 | 22.6 | 31.8 | 11.6 | 3.3 | 3.1 | |
| Dehydrolucidine B | - | - | 3.1 | 3.8 | 4.7 | 3.6 | 4.5 | 5.4 | 5.7 | 4.7 | 2.5 | 3.2 | 2.5 | 4.2 | 14.3 | 41.7 | 24.5 | 5.6 |
| Oxolucidine B | 61.2 | 18.6 | 12.3 | 11.7 | 9.7 | 11.5 | 8.7 | 6.6 | 7.1 | 3.7 | 2.1 | 3.1 | 2.3 | 1.9 | - | - | - | - |
| Unidentified 440.3637 C28H46N3O |
- | - | - | - | - | - | - | - | - | - | - | - | - | - | 5.7 | 10.8 | 2.8 | 17.9 |
| Unidentified 456.3588 C28H46N3O2 |
- | - | - | - | - | - | - | - | - | - | - | - | - | - | 1.2 | 2.9 | 3.3 | - |
| SUM % | 67.5 | 72.9 | 90.8 | 77.1 | 93.5 | 96.1 | 85.6 | 91.9 | 89.7 | 85.2 | 89.8 | 95.6 | 91.2 | 98.7 | 93.1 | 93.7 | 88.8 | 80.3 |