Table 6.
Results of alkaloids identified via HPLC/ESI-QTOF-MS present in fractions 1–16 obtained from experiment 2 via VLC using sorbents Al2O3 and silica gel in ratio 3:1 from H. selago extract.
| Isolated Compounds | Fraction Number | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | |
| Luciduline | 3.5 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| Serratidine | 2.2 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| Selagoline | - | 2.9 | 7.3 | 19.0 | 26.4 | 23.5 | 22.6 | 14.8 | 22.4 | 23.2 | 27.2 | 18.2 | 8.0 | 1.2 | - | - |
| Lycopodine N-oxide | - | 9.5 | 43.6 | 26.8 | 30.5 | 25.7 | 24.1 | 23.8 | 24.1 | 26.8 | 24.1 | 15.1 | 28.8 | 41.6 | 10.9 | - |
| Dehydrooxolucidine B | 67.9 | 50.1 | 18.1 | 25.7 | 11.2 | 18.2 | 19.6 | 20.3 | 29.2 | 12.2 | 6.2 | 9.4 | - | - | 21.1 | 43.1 |
| Lucidine B | 6.6 | 30.1 | 24.0 | 24.2 | 22.6 | 20.2 | 23.1 | 27.1 | 17.2 | 29.2 | 32.3 | 33.2 | 13.6 | - | - | 1.1 |
| Dehydrolucidine B | - | - | - | - | 1.9 | 4.8 | 3.2 | 7.0 | 4.1 | 1.9 | 1.2 | 1.6 | 25.2 | 15.2 | 4.1 | 1.4 |
| Oxolucidine B | 17.7 | 4.1 | 3.0 | 2.7 | 2.6 | 2.2 | 2.1 | 1.1 | 1.4 | 2.0 | 0.2 | 1.1 | 0.8 | - | - | - |
| Lycodine | 0.9 | 0.6 | 0.7 | 0.5 | 0.8 | 0.4 | 0.9 | 0.4 | 0.6 | - | - | - | - | - | - | |
| Lycoposerramine B | 1.1 | 1.1 | 1.1 | - | 0.9 | 1.1 | - | - | - | - | - | - | - | - | - | - |
| Fawcettimine | - | - | - | - | - | - | - | - | - | - | - | - | - | 8.3 | - | - |
| Dihydrolycopodine | - | - | - | - | - | - | - | - | - | - | - | - | 3.2 | 1.5 | 15.2 | 14.2 |
| Deacetyllycoclavine | - | - | - | - | - | - | - | - | - | - | - | - | - | - | 3.1 | 7.1 |
| 8-β,11-α Dihydroxy-lycopodine | - | - | - | - | - | 0.8 | 0.9 | 0.6 | 0.9 | 1.0 | 1.0 | 2.2 | 5.2 | 4.2 | 5.3 | 4.2 |
| Huperzine A | - | - | - | - | - | - | - | - | - | - | - | 8.1 | 6.6 | 0.9 | ||
| Unidentified 474.3796 C27H48N5O2 or C26H52NO6 |
- | - | - | - | - | - | - | - | - | - | - | - | - | - | 8.2 | 10.1 |
| Unidentified 426.3843 C28H48N3 or C30H46O2 |
- | - | - | - | - | - | - | - | - | - | - | - | - | 6.5 | - | - |
| Unidentified 440.3637 C28H46N3O |
- | - | - | - | - | - | - | - | - | - | - | - | 6.1 | 9.9 | 2.3 | 3.8 |
| Unidentified 456.3588 C28H46N3O2 |
- | - | - | - | - | - | - | - | - | - | - | - | 2.4 | 1.4 | 6.1 | - |
| Unidentified 438.3481 C28H44N3O |
- | - | - | - | - | - | - | - | - | - | - | - | - | 4.1 | 4.3 | - |
| SUM % | 98.9 | 98.7 | 97.6 | 99.0 | 96.4 | 97.2 | 96.1 | 95.4 | 99.7 | 96.8 | 92.2 | 88.8 | 99.7 | 94.8 | 80.7 | 84.8 |