Table 7.
Results of alkaloids identified via HPLC/ESI-QTOF-MS present in fractions 1–6 obtained from experiment 1 via VLC using sorbents Al2O3 and silica gel in ratio 1:3 from L. clavatum extract.
| Isolated Compounds | Fraction Number | |||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | |
| Acetyllycofawcine | - | - | - | 10.0 | 13.9 | - |
| Acetylfawcettiine | - | - | - | 12.2 | 10.9 | - |
| Lycopodine N-oxide | - | - | 9.1 | 15.5 | 13.3 | 20.6 |
| Lycopodine | - | - | 7.4 | 7.5 | 8.1 | - |
| Flabelline | - | - | - | 3.9 | 2.6 | - |
| Fawcettimine | - | - | - | 1.5 | 0.8 | 5.7 |
| Deacetyldidehydrolycofawcine | - | - | - | - | 6.9 | - |
| Lycodine | - | 3.9 | 3.9 | 1.4 | - | - |
| 4α,8β,12β-Trihydroxylycopodine | - | - | 4.3 | - | - | - |
| 4α,6α-Dihydroxyanhydrolycodoline | - | - | - | 1.3 | - | - |
| 4,6α-Dihydroxylycopodine or epimer | - | - | - | - | 5.3 | - |
| Lycoposerramine K | 12.3 | 0.8 | - | - | - | - |
| Lycofawcine | - | - | - | - | - | 8.5 |
| α-Lofoline or epimer | - | - | - | - | 0.7 | 5.4 |
| Deacetylfawcettiine | - | - | - | - | - | 2.3 |
| 8β-Hydroxylycoposerramine K | - | - | - | - | - | 5.7 |
| 16-Oxolyclanitin | - | - | - | 5.9 | - | 0.3 |
| Lycoclavanin or epimer | - | - | 4.9 | 7.6 | - | - |
| Serratezomine E | - | - | - | - | - | 4.0 |
| Huperzinine | 0.4 | 2.8 | 3.2 | - | - | - |
| Unidentified 371.2290 C17H31N4O5 or C19H33NO6 |
12.0 | 12.6 | 11.1 | 5.5 | 7.2 | 1.4 |
| Unidentified 415.2532 C19H35N4O6 |
12.5 | 13.2 | 11.7 | 6.6 | 8.4 | 4.9 |
| Unidentified 679.5133 C40H71O8 or C41H67N4O4 |
9.0 | 8.3 | 6.9 | 4.5 | 1.1 | 5.7 |
| SUM % | 46.2 | 41.5 | 62.6 | 83.4 | 79.0 | 64.4 |