Table 8.
Results of alkaloids identified via HPLC/ESI-QTOF-MS present in fractions 1–10 obtained from experiment 2 via VLC using sorbents Al2O3 and silica gel in ratio 3:1 from L. clavatum extract.
| Isolated Compounds | Fraction Number | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | |
| Acetyllycofawcine | 7.08 | - | - | 4.39 | 4.4 | 3.29 | 2.48 | 4.97 | 3.17 | 3.19 |
| Acetylfawcettiine | 10.31 | 6.98 | 4.89 | 7.03 | 7.53 | 7.55 | 6.17 | 7.26 | 3.45 | 2.92 |
| Lycopodine N-oxide | 5.57 | 7.03 | 7.28 | 5.16 | 7.46 | 5.26 | 6.71 | 5.03 | 6.88 | 6.24 |
| Lycopodine | - | 2.99 | 2.37 | 3.06 | 3.3 | 5.01 | 8.75 | 6.99 | 6.67 | 4.64 |
| Flabellidine | 0.78 | - | - | - | - | - | 1.13 | 1.01 | 2.14 | 2.03 |
| Fawcettimine | - | - | - | - | - | - | - | - | 1.15 | 6.25 |
| Lycodine | 3.78 | 1.61 | 1.38 | 3.67 | 1.46 | 1.96 | 1.83 | 2.96 | 1.91 | 0.93 |
| Lycoposerramine K | 3.88 | 1.95 | 0.69 | 0.78 | 0.75 | 0.55 | - | - | - | - |
| α-Lofoline or epimer | - | - | - | - | - | - | - | - | 1.1 | 3.3 |
| Serratezomine E | 4.7 | 6.59 | ||||||||
| Deacetylfawcettiine | - | - | - | - | - | - | - | - | - | 2.37 |
| Lycofawcine | 1.12 | 0.89 | ||||||||
| Huperzinine | 2.73 | 0.45 | 0.55 | 2.08 | 2.09 | 2.81 | 2.15 | 2.62 | - | - |
| 16-Oxolyclanitin | 0.77 | 1.24 | ||||||||
| Lycoclavanin or epimer | 3.31 | 2.11 | 3.12 | 3.75 | 2.41 | 3.15 | 2.13 | 0.88 | ||
| Unidentified 371.2290 C17H31N4O5 or C19H33NO6 |
9.74 | 12.05 | 12.1 | 9.48 | 9.48 | 8.06 | 8.55 | 8.07 | 8.29 | 7.09 |
| Unidentified 415.2532 C19H35N4O6 |
10.51 | 12.77 | 12.67 | 9.63 | 9.8 | 8.68 | 8.87 | 8.23 | 7.67 | 7.57 |
| Unidentified 679.5133 C40H71O8 or C41H67N4O4 |
6.46 | 7.89 | 7.47 | 8.01 | 8.48 | 6.94 | 6.24 | 5.93 | 4.94 | 4.6 |
| SUM % | 64.15 | 55.83 | 52.52 | 57.04 | 57.16 | 53.26 | 55.01 | 53.95 | 53.96 | 59.85 |