Table 10.
Results of alkaloids identified via HPLC/ESI-QTOF-MS present in fractions 1–11 obtained from experiment 4 via VLC using silanized silica gel as a sorbent and reverse phase system from L. clavatum extract.
| Isolated Compounds | Fraction Number | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | |
| Lycopodine | 0.8 | - | 28.5 | 31.8 | 27.6 | 30.0 | 33.5 | 39.1 | 36.8 | 35.4 | 37.2 |
| Dihydrolycopodine | - | - | 8.2 | 12.3 | 12.7 | 8.5 | 6.2 | 6.0 | 7.6 | 11.3 | 7.0 |
| Lycopodine N-oxide | - | - | - | 8.5 | 11.8 | 13.0 | 10.1 | 8.8 | 11.0 | 13.2 | 13.2 |
| 8β-Hydroxylycoposerramine K | - | 46.3 | 22.8 | 13.3 | 8.9 | 7.3 | 3.7 | 4.8 | 3.2 | 5.5 | 4.3 |
| Acetylfawcettiine | - | - | - | - | - | - | 1.1 | 0.9 | - | 0.8 | 1.1 |
| 8-β,11-α Dihydroxylycopodine or epimer | - | - | 3.9 | - | - | 1.0 | - | 1.2 | 0.9 | 0.7 | 1.0 |
| Lycodine | - | - | - | 1.2 | 1.1 | 0.8 | - | - | 1.1 | 0.9 | 0.6 |
| α-Lofoline or epimer | - | - | - | 1.1 | 1.1 | 1.0 | 1.1 | 1.1 | 2.0 | 1.0 | 1.2 |
| β-Obscurine | - | - | - | 1.2 | 2.1 | 1.2 | - | - | - | - | 1.1 |
| Deacetylfawcettiine | - | 1.1 | 4.6 | 3.2 | 3.5 | 4.6 | 2.2 | 1.7 | 3.6 | 1.9 | 3.4 |
| Serratezomine E | - | - | - | 1.5 | 2.9 | 1.5 | 1.0 | 1.3 | 1.2 | 3.2 | 3.6 |
| N-Methyl lycodine | - | - | - | - | - | - | - | - | 0.5 | 0.6 | 0.8 |
| Unidentified 236.1489 C10H22NO5 |
- | 20.1 | 11.7 | 10.3 | 9.8 | 10.0 | 9.3 | 9.9 | 11.1 | 12.5 | 11.5 |
| SUM % | 0.8 | 67.5 | 79.8 | 84.4 | 81.4 | 78.7 | 68.0 | 74.7 | 79.0 | 87.0 | 85.8 |