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. Author manuscript; available in PMC: 2022 Sep 14.
Published in final edited form as: Mol Genet Metab. 2021 Sep 14;134(1-2):156–163. doi: 10.1016/j.ymgme.2021.09.002

Figure 6. Oroboros high-resolution respirometry of isolated heart mitochondria from wild type and heart specific Acad9−/− mice.

Figure 6.

Isolated mitochondria were added to the assay media and oxygen consumption was measured after the addition of malate and pyruvate (mal+pyr), adenosine diphosphate (ADP), glutamate (Glu), succinate (Suc), and rotenone (Rot). Robust oxygen consumption due to complex I (CI) and complex II (CII) was evident in wild type mitochondria, while only complex II activity was present in ACAD9−/− mitochondria.