Figure 2.

Screening of synthesized aptamer motifs. Synthesized biotinylated motifs of V3 and V5 were incubated with IGROV cells. Fluorescein isothiocyanate (FITC) conjugated streptavidin is used to detect biotinylated motifs. The binding affinity of the aptamer motifs was assessed based on the fluorescence intensity using flow cytometry. Histogram graphs demonstrated the fluorescence intensity of V3 aptamer motifs (A), and V5 aptamer motifs (B) binding to IGROV cells. Vimentin expression was evaluated and confirmed using an anti-human vimentin antibody before screening the binding affinity of motifs (C). A scrambled aptamer was used as a control.