Figure 5.

Validating specific binding of selected motifs with the human cell line. Vimentin-expressing IGROV cells were incubated with biotinylated V3M2, V5M2, anti-human vimentin antibody (VIM Ab) or scrambled control (SA) aptamer. Fluorescein isothiocyanate (FITC) conjugated streptavidin was used to detect biotinylated aptamers. The binding proficiencies were determined by fluorescence intensity using fluorescence microscopy. Fluorescence intensity is quantified by normalizing the fluorescence intensity of pixel per area (intensity of motif/intensity of DAPI) and presented as a bar graph with mean ± SE of three replicates. Hoechst 33,342 was used to stain the cell nuclei (blue).