Figure 6.

Detection of vimentin expression in human ovarian tumor tissue. Tissue sections of human ovarian tumor or normal ovarian tissue were incubated with biotinylated V3M2 or V5M2 at a concentration of 250 nM, followed by streptavidin-FITC to detect their binding affinity. Anti-human vimentin antibody was also used as a positive control for both ovarian tumor tissue and normal ovarian tissue. Images are representative of three samples of ovarian tumor or normal ovarian tissue. Fluorescence intensity is quantified by normalizing the fluorescence intensity of pixel per area (intensity of motif/intensity of DAPI) and presented as a bar graph with mean ± SE of three replicates. Hoechst 33,342 was used to stain the cell nuclei (blue).