Figure 5.

RV rescues the mitochondrial membrane potential in aged porcine oocytes. Representative Fluorescent images of JC-1-stained oocytes. Cultured in the absence or presence of 2 µmol/L RV. (a,f) Control; (b,g) aged 24 h; (c,h) aged 24 h + RV; (d,i) aged 48 h; (e,j) aged 48 h + RV. (k) Quantitative analysis of JC-1 red/green fluorescence intensity ratio in porcine oocytes. Membrane potential was calculated as the ratio of red fluorescence, which corresponds to activated mitochondria (J-aggregates), and to green fluorescence, which corresponds to less-activated mitochondria (J-monomers). Fluorescence emitted from each oocyte was analyzed using the ImageJ software. Significant difference (* p < 0.05).