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. 2021 Oct 31;26(21):6617. doi: 10.3390/molecules26216617

Figure 3.

Figure 3

RNA stabilization buffer improved one-step RT-LAMP amplification. (A) A viral RNA diluted in heat-inactivated saliva from healthy individuals was mixed in 1:1 ratio with either water, sample buffer, sample buffer with RNAsecure or Chelex, or RNA stabilization buffer; heat-treated; and RT-LAMP-amplified (n = 6). (B) The RT-LAMP reaction mix with guanidine chloride was used to amplify RNA (315 copies per reaction) (n = 12). (A,B) RNA was amplified with N2 primers in 20 µL reaction volume using a WarmStart Mix.