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. 2021 Oct 31;26(21):6617. doi: 10.3390/molecules26216617

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Longitudinal analysis of SARS-CoV-2 virus in infected individuals and hospitalized patients. (A,B) Sequential saliva sampling and detection of SARS-CoV-2 infection. Two households are shown. The appearance of COVID-19 symptoms is marked with a vertical dashed line, and a red arrow indicates the day of a positive NPS-RT-qPCR test. The viral infection among individuals is schematically presented at the top. (C,D) Saliva collected from hospitalized COVID-19 patients was analyzed for the SARS-CoV-2 virus. Results grouped as positive and negative for SARS-CoV-2 in saliva are presented in relation to the time between symptoms or hospital admission and saliva tests. (A–D) Saliva samples were analyzed with saliva-based one-step RT-LAMP or RT-qPCR, and combined results were presented.