Table 2.

Validation results of the analytical factors of HPLC method.

Analytical Factor	GSH	GSSG
LLOQ (µg/g brain)	0.50
rLLOQ (%)	97.11	98.86
LLOQrec (µg/g brain)	0.50	0.50
rLLOQrec (%)	85.47	108.78
Slope	1562	2124.4
Y-intercept	−350.11	−1493.4
Determination coefficient (r2)	0.997	0.996
Analytical range (µg/g brain)	0.50–50
Retention time	6.22 ± 0.06

rLLOQ, relative lower limit of quantification; LLOQrec, recovery corrected LLOQ; rLLOQrec (%), relative recovery-corrected LLOQ;