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. 2021 Nov 12;434(2):167357. doi: 10.1016/j.jmb.2021.167357

Figure 4.

Figure 4

Affinity determination of the RBD – ACE2 interaction in the presence of disulfide-reducing compounds at 37 °C by microscale thermophoresis (MST). The graph shows the change in baseline-corrected normalized fluorescence (delta Fnorm (‰)) of labeled RBD as a function of ACE2 concentration. The graph shows a sigmoidal binding curve with a plateau at high ACE2 concentrations for the negative control, oxidized DTT, NAC and GSH. For DTT and TCEP the plateau at high ACE2 concentrations is absent, indicating weaker binding.