Figure 5.

Virus titer and cell viability as a function of the concentration of (A) DTT, (B) TCEP, (C) GSH, (D) NAC and (E) carboxymethyl-cysteine. The figure shows data from a representative experiment. The viral titer, expressed as a TCID₅₀/ml (left Y-axis) of SARS-CoV-2 and the viability of Vero’76 cell (% of the viability of untreated cell control) (right Y-axis), determined by the MTS assay, are plotted as the function of compound concentration (mM) (X-axis). The insets show the viability of Vero’76 cell, determined by the resazurin assay. Virus titers are colored in blue, while the viability data is colored in red for the MTS assay and purple for the resazurin assay. Because X-axis is logarithmic, viral titers and cell viability at 0 mM compound concentration are represented at 0.05 mM concentration for DTT and TCEP and at 0.2 mM concentration for GSH, NAC and carboxymethyl-cysteine.