TABLE 2.

Oligonucleotide primers synthesized in this study

Primer purpose and name	Sequence	Nucleotide position
Universal primers for 23S rRNA gene
ER-1F	5′-CACGGTGGATGCCTTGGC-3′	1186–1203
ER-2R	5′-CCTTTCCCTCACGGTACTG-3′	1654–1672
ER-3F	5′-GCGTGCCTTTTGTAGAATG-3′	1755–1773
ER-4R	5′-AGTGAGCTATTACGCACTCTTT-3′	2282–2303
ER-5F	5′-GCTCGTCCGCTCAGGGTTAG-3′	2571–2589
ER-6R	5′-CATTTTGCCGAGTTCCTTAA-3′	2861–2880
ER-7F	5′-GTTAAGGAACTCGGCAAAATG-3′	2860–2881
ER-8R	5′-GTTACGGCCGCCGTTTACTGG-3′	3062–3082
ER-9F	5′-AGTTCCGACCCGCACGAAAGG-3′	3172–3192
ER-10R	5′-AGGCGACCGCCCCAGTCAAAC-3′	3460–3480
Primers for sequencing of 23S rRNA gene
ER1 · 2F	5′-TCTAGTGGTATCCTGAGTACG-3′	1554–1574
ER3 · 4R	5′-TTCGGGTCTACCACAATGTAC-3′	1855–1875
ER3 · 4F	5′-CGTAGTCGAAAGGGAAACAGC-3′	2163–2183
ER5 · 6R	5′-TAAACCAGCACTTCCAGTCGC-3′	2644–2664
ER5 · 6F	5′-GATGCCAGCTCTCAAGAAAAG-3′	2752–2772
ER7 · 8R	5′-GTCTTGCGACTTTGCAGAGAG-3′	2959–2979
ER7 · 8F	5′-AGTCGCAAGACGAAGTATAGG-3′	2969–2989
ER9 · 10R	5′-CTAAGATTTCACCGAGTCTGC-3′	3169–3189
Primers for LA PCR cloning
ER-S1	5′-TGCTTGTGTACGTACCACTCC-3′	1248–1265
ER-S2	5′-GCCATTGGGATACCACTCTTG-3′	3330–3350
Primers for sequencing of rRNA gene cluster
ER-S3	5′-CAAGGTATCCCTACCGGAAG-3′	817–836
ER-S4	5′-GGAGCTGAATTAGGTTCCAAG-3′	3691–3711
ER-S5	5′-CACCCTCTAATCGATATGCATC-3′	4567–4588
ER-S6	5′-GTGTAGCGGTAAAATGCGTAG-3′	9–29
ER-S7	5′-GAGACTGCCGGTGATAAACC-3′	467–486
ER-S8	5′-CTCGCCCAAGAGTTCACATC-3′	3629–3648
ER-S9	5′-GATATTGTCGCCGCTAAAGTG-3′	4200–4220