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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Bioorg Chem. 2021 Sep 8;116:105340. doi: 10.1016/j.bioorg.2021.105340

Figure 2:

Figure 2:

PCS2D1.2 selectively binds to and inhibits the ALDH+ CSCs in lung cancer. (A) The CSC cells (ALDH+) and non-CSCs (ALDH−) from H358 cells were sorted using ALDH activity based commercial assay kit. qRT-PCR was performed to determine the expression of cancer stem cell markers plectin, ALDH1A3 and SOX2 in ALDH+ vs ALDH− cells. (B) ALDH+ cells and ALDH− cells were incubated with biotinylated-PCS2D1.2 and probed with streptavidin Q-dots 655 and visualized with fluorescent-microscope under long pass DAPI filter. (C) Anti-proliferative effects of PCS2D1.2 on ALDH+ CSCs and ALDH− non-CSCs from H358 cell line were determined using MTS assay.