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. 2021 Oct 30;23(5):576–583. doi: 10.22074/cellj.2021.7352

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The Cell Journal (Yakhteh) is an open access journal which means the articles are freely available online for any individual author to download and use the providing address. The journal is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported License which allows the author(s) to hold the copyright without restrictions that is permitting unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig.1 — The morphology and cell profile markers of human dermal papilla cells (hDPCs). A. The morphology and cell profile markers of hDPC. Aa. hDPC showed a typical spindle-like morphology of fibroblast cell. Ab. Alkaline phosphatase activity (red) showed in hDPC passage two into a conventional culture medium. Ac. Dermal papilla colony tested strongly positive for toluidine blue staining (red). Ad. Immunofluorescent Imaging data was presented as α-SMA expression after culture (green). Ae. Nuclei were stained with DAPI (blue) and Af. Pseudo colored merged image. Ag-Ai. Secondary antibody control in hDPC. As a negative control, staining was performed without primary antibody, showing non-specific staining by the secondary antibody (Alexa Fluor 488 goat anti-mouse, (control). Ak. Immunofluorescent Imaging data was presented as versican expression after culture (green). Al. Nuclei were stained with DAPI (blue) and Am. Pseudo colored merged image. An-Ap. Secondary antibody control in hDPC. As a negative control, staining was performed without primary antibody, showing non-specific staining by the secondary antibody (Alexa Fluor 488 goat anti-rabbit, (control). Flow data are presented as dot plot and histograms from the specific markers (blue) overlaid with control (red) from hDPC. The dot plot and histogram flow cytometry analysis illustrated that hDPC were positive for B. α-SMA and C. Versican (scale bars: 200 μm, n=3).