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. 2021 Oct 30;23(5):576–583. doi: 10.22074/cellj.2021.7352

Fig.2.

Fig.2

The morphology and cell profile markers of outer root sheet cells (ORSCs). A. The morphology and cell profile markers of ORSCs. Aa. ORSCs showed a typical morphology of polygonal-shaped structures. Ab. Immunofluorescent imaging data was presented as K15 expression after culture (green). Ac. Nuclei were stained with DAPI (blue) and Ad. Pseudo colored merged image. Ae-g. Secondary antibody control in human dermal papilla cells (hDPCs). As a negative control, staining was performed without primary antibody, showing non-specific staining by the secondary antibody [Alexa Fluor 488 Goat anti-mouse (control)]. B. Flow data is presented as histograms from specific markers (blue) overlaid with control (red) from ORSCs. The histograms of flow cytometry analysis illustrated that ORSCs were positive for K15 (scale bars: 200 μm, n=3).