Fig.3.

Characterization of exosomes derived from ADSc and PRP. A, B. SEM evaluation of exosomes (scale bars: 2 μm). C, D. Nanoparticle evaluation of ASC-Exo and PRP-Exo. The mean diameter of ASC-Exo and PRP-Exo were 167 and 147 nm. E, F. Immunoblotting for CD63, CD81, CD9, TSG101 and Calnexin in exosomes. G. Internalization of exosomes by human dermal papilla: confirmation of the uptake of exosomes in hDPC. The PKH26 (red) kit (20 μg/mL) were applied to stain ASC-Exo or PRP-Exo and incubated with hDPC for 24 hours (scale bars: 200 μm). PBS was used as negative control (NC). Nuclei were stained with DAPI (blue) for counterstaining (n=3). ADSc; Adipose-derived stem cells, PRP; Platelet-rich plasma, ASC; Adipose stromal cell exosome, hDPC; Human dermal papilla cell, and PBS; Phosphate-buffered saline.