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. 2021 Oct 30;23(5):516–522. doi: 10.22074/cellj.2021.7849

Table 1.

Characteristics of specific primers used for reverse transcription- quantitative polymerase chain reaction (RT-qPCR)


Genes Oligonucleotide sequences (5ˊ-3ˊ) PCR product size (bp) Tm (˚C) Accession number

TLR4 F: ACCTGGCTGGTTTACACGTC 201 60 NM_010477
R: CTGCCAGAGACATTGCAGAA
GAPDH F: CCATCCGGGTTCCTATAAAT 198 54 NM_008084
R: AATCTCCACTTTGCCACTG