Extended Data Fig. 4 |. Analysis of cryo-EM densities and models.

a, Half-map-to-half-map and model-to-map Fourier shell correlation (FSC) for the 33-fold symmetric GSDMD pore and prepore from the L192E dataset. Horizontal dashed lines represent FSC cut-offs at 0.5 and 0.143. b, c, Pore-form (b) and prepore-form (c) GSDMD subunits fitted into their respective cryo-EM density. Arrows indicate secondary structural elements specified by residue numbers. d, Close-up views of the pore-form GSDMD structure fitted into its cryo-EM density at six representative locations denoted by residue numbers.