TABLE 1.
Nature and frequency of amino acid substitutions at resistance-associated codons in the RT gene: cross-sectional analysis of 287 sequences
| Mutation at the following codona:
| ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 41 (M) | 62 (A) | 65 (K) | 67 (D) | 69 (T) | 70 (K) | 74 (L) | 75 (V) | 103 (K) | 106 (V) | 151 (Q) | 181 (Y) | 184 (M) | 188 (Y) | 210 (L) | 215 (T) | 219 (K) |
| L (98) | V (1) | —b | N (66) | D (20) | R (59) | I (4) | L (1) | R (5) | I (5) | — | N (1) | V (75) | L (1) | W (73) | Y (82) | Q (36) |
| I (1) | G (1) | N (15) | A (2) | V (2) | A (1) | E (1) | C (1) | I (1) | S (2) | F (12) | N (8) | |||||
| E (1) | A (7) | G (2) | A (1) | N (1) | D (1) | F (1) | D (5) | E (4) | ||||||||
| L (1) | S (6) | S (1) | G (1) | I (5) | R (4) | |||||||||||
| S (1) | E (2) | N (5) | D (2) | |||||||||||||
| C (1) | C (3) | |||||||||||||||
| I (1) | c (26) | |||||||||||||||
| K (1) | ||||||||||||||||
| L (1) | ||||||||||||||||
| Y (1) | ||||||||||||||||
a
A mutation was considered when it was unambiguously detected in the electropherogram of the sequenced sample. The letters in parentheses are the wild-type amino acid. The numbers in parentheses refer to the number of sequences with the indicated mutation.
b
—, none.
c
In the case of codon 215, mutations could be detected at both the first and the second positions of the codon (Fig. 2).