Table 3.

Pathological alteration of the liver in H&E and reticulin staining

Number	Pathological signs	Control	Vehicle	Acetaminophen	Saffron 150	Saffron 300
H&E staining
1	Glycogen depletion	0	0	1*	0	0
2	Congestion	0	0	3***	1	1
3	Sinusoidal dilation	0	0	2***	1	1
4	Inflammatory infiltration (Lymphocytic infiltration)	0	0	2	1	0
5	Vacuolization	0	0	3***	1*	0
6	Bile stasis	0	0	2*	0	0
7	Bile plugs	0	0	1	0	0
8	Kuffer cell hyperplasia	0	0	2*	2*	3***
9	Pyknosis	0	0	1	0	0
10	Necrosis	0	0	2*	0	0
Reticulin staining
Reticulin fibers		0	0	2***	0###	0###

Histological changes were scored as none (0), active damage less than 25% (+), active damage less than 50% (++), active damage less than 75% (+++) damage and active. *Significant differences from the control group (*p≤0.05, **p≤0.01 and ***p≤0.001); #significant differences from the acetaminophen-treated group (###p≤0.001).