| Antibodies |
| SCAI |
Abcam |
Ab124688 |
| Mouse anti-Vinculin |
Sigma |
V9131 |
| Mouse monoclonal anti-Flag |
Sigma |
F1804 |
| Rabbit monoclonal anti-Flag |
Sigma |
F7425 |
| Anti-BrdU antibody [BU1/75 (ICR1)] |
Abcam |
Ab6326 |
| GFP |
Abcam |
ab290 |
| ATM |
Abcam |
ab32420 |
| FANCA |
Bethyl |
A301-980 |
| FANCI |
Bethyl |
A301-254 |
| FANCD2 |
Novus |
NB100-182 |
| 53BP1 |
Bethyl |
A300-272A |
| BRCA2 |
Bethyl |
A300-005A |
| SMARCAL1 |
Santa Cruz |
sc-376377 |
| ZRANB3 |
Bethyl |
A303-033A |
| HELB |
Abcam |
ab202141 |
| SLX4 |
Bethyl |
A302-270A |
| ERCC1 |
Cell Signaling |
3885S |
| SNM1A |
Bethyl |
A303-747A |
| FAAP24 |
GeneTex |
GTX117277 |
| RNAseH2A |
Abcam |
ab92876 |
| REV7 |
Santa Cruz |
sc-135977 |
| CtIP |
Bethyl |
A300-488A |
| MRE11 |
Cell Signaling |
4895 |
| MUS81 |
Santa Cruz |
sc-53382 |
| ORC2 |
Abcam |
ab68348 |
| DNA2 |
Abcam |
ab96488 |
| EXO1 |
Bethyl |
A302-640A |
| RPA2-P-S4/8 |
Bethyl |
A300-245A |
| RPA32 |
GeneTex |
GTX70258 |
| CHK1-P-S345 |
Cell Signaling |
2348S |
| CHK1 |
Santa Cruz |
Sc-8408 |
| γH2AX |
Millipore |
05-636 |
| H2AX |
Bethyl |
A300-082A |
|
|
|
| Bacterial and virus strains |
| Lenti-Blast-3Flag FANCM WT |
Wang et al, 2018
|
N/A |
| Lenti-Blast-3Flag FANCM K117R |
Wang et al, 2018
|
N/A |
| LentiCrispr v2 |
Sanjana et al, 2014
|
Addgene #52961 |
|
|
|
| Biological samples |
|
|
|
|
|
|
|
|
| Chemicals, peptides, and recombinant proteins |
| DMSO |
Amresco |
N182 |
| Hydroxyurea |
Sigma |
H8627-10G |
| Mitomycin C (MMC) |
Santa Cruz Biotech |
M4287-2MG |
| Cisplatin (cisp) |
HMS Ludwig |
N/A |
| Mirin |
Sigma |
M9948 |
| Olaparib |
Selleck chemicals |
S1060 |
| 4-hydroxytamoxifen |
Sigma |
H7904-5MGG |
| α-Amanitin |
Abcam |
ab144512 |
| 5,6-Dichlorobenzimidazole 1-β-D-ribofuranoside (DRB) |
Sigma |
D1916 |
| RNA Polymerase III inhibitor (ML-60218) |
Sigma |
557403 |
| Superscript IV reverse transcriptase |
Invitrogen |
18090050 |
| Lipofectamine RNAiMAX |
Invitrogen |
13778075 |
| Lipofectamine 3000 |
ThermoFisher |
L3000008 |
| TransIT®-LT1 Transfection Reagent |
Mirus |
MIR2300 |
| 5-Chloro-2′-deoxyuridine (CIdU) |
Sigma |
C6891 |
| 5-Iodo-2′-deoxyuridine (IdU) |
Sigma |
I7125 |
| cOmplete, Mini, EDTA-free, Protease Inhibitor Cocktail Tablets |
Roche |
11836170001 |
| Phosphatase Inhibitor Cocktail Set I |
Sigma |
524624 |
| Phosphatase Inhibitor Cocktail Set II |
Sigma |
524625 |
| Halt Protease and Phosphatase Inhibitor Cocktail (100X) |
Fisher |
78440 |
|
|
|
| Critical commercial assays |
| RNeasy Plus mini kit |
Qiagen |
74134 |
| Taqman Gene expression Mastermix |
Life Technologies |
4369016 |
| Taqman Gene Expression Assay Human REV1 |
ThermoFisher Scientific |
Catalog # 4331182 Assay ID: Hs00249411_m1 |
| Taqman Gene Expression Assay Human REV3L |
ThermoFisher Scientific |
Catalog # 4331182 Assay ID: Hs00161301_m1 |
| Taqman Gene Expression Assay Human GAPDH |
ThermoFisher Scientific |
Catalog # 4331182, Assay ID:Hs99999905_m1 |
| AlamarBlue |
ThermoFisher |
DAL1025 |
| CellTitre-Glo 2 |
Promega |
G9242 |
| Click-iT™ Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 594 dye |
ThermoFisher |
C10639 |
| APC BrdU Flow kit |
BD biosciences |
552598 |
| Quickchange II XL Site Directed Mutagenesis Kit |
Agilent |
200521 |
|
|
|
| Deposited data |
|
http://dx.doi.org/10.17632/35f2n4wsnv.1
|
|
|
|
|
|
| Experimental models: Cell lines |
| Human: U2OS |
ATCC |
N/A |
| Human: U2OS SCAI KO |
This paper |
N/A |
| Human: U2OS WT FANCI |
Ishiai et al, 2008
|
N/A |
| Human: U2OS DN FANCI-1 |
Ishiai et al, 2008
|
N/A |
| Human: U2OS DN FANCI-2 |
Ishiai et al, 2008
|
N/A |
| Human: ER-AsiSi U2OS |
Zhou et al, 2014
|
N/A |
| Human: REV7 KO U2OS |
Bluteau et al, 2017
|
N/A |
| Human: HeLa |
ATCC |
N/A |
| Human: RPE |
ATCC |
N/A |
| Human: 293T |
ATCC |
N/A |
| Mouse: 6xTer/HR ES cells |
Willis et al, 2014
|
N/A |
|
|
|
| Experimental models: Organisms/strains |
|
|
|
|
|
|
| Oligonucleotides |
| Listed in Table S7
|
|
|
|
|
|
| Recombinant DNA |
|
|
| REV3L (NM_002912) Human Tagged ORF Clone |
Origene |
RG222943 |
| pETDuet1-(R)-hREV3 |
Tomida et al, 2015
|
Addgene #64872 |
| GFP-REV3 |
This paper |
N/A |
| Flag-SCAI |
This paper |
N/A |
| FLAG-HA-SCAI |
This paper |
N/A |
| GFP-SCAI |
This paper |
N/A |
|
|
|
| Software and algorithms |
|
|
| MaGeck |
Li et al, 2014
|
https://sourceforge.net/p/mageck/wiki/Home/
|
| EdgeR |
Robinson et al, 2010
|
https://bioconductor.org/packages/release/bioc/html/edgeR.html
|
| Flowjo |
N/A |
https://www.flowjo.com/
|
| ImageJ |
N/A |
https://imagej.nih.gov/ij/
|
| Graphpad Prism |
N/A |
https://www.graphpad.com/scientific-software/prism/
|
|
|
|
| Other |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
